Packaging and storage—
Preserve in well-closed containers.
Identification—
A:
A solution (1 in 10) responds to the tests for
Calcium 
191
.
B:
To a solution of 0.5 g in 5 mL of water add 5 mL of 1 N sodium hydroxide, and filter. To the filtrate add 5 mL of iodine TS: a precipitate of iodoform is produced.
pH 791:
between 7.0 and 8.5, in a solution (1 in 10).
Loss on drying 731—
Dry it at a pressure not exceeding 5 mm of mercury at 60
for 5 hours: it loses between 10.5% and 12.0% of its weight.
Chloride 221—
A 1.0-g portion shows no more chloride than corresponds to 1.0 mL of 0.020 N hydrochloric acid (0.07%).
Sulfate 221—
A 2.0-g portion shows no more sulfate than corresponds to 1.0 mL of 0.020 N sulfuric acid (0.05%).
Reducing sugars—
Dissolve 0.50 g in 10 mL of water, add 2 mL of 3 N hydrochloric acid, boil for about 2 minutes, and cool. Add 5 mL of
sodium carbonate TS, allow to stand for 5 minutes, dilute with water to 20 mL, and filter. Add 5 mL of the clear filtrate to about 2 mL of
alkaline cupric tartrate TS, and boil for 1 minute: no red precipitate is formed immediately.
Assay—
Dissolve about 600 mg of Calcium Levulinate, accurately weighed, in 150 mL of water containing 2 mL of 3 N hydrochloric acid. While stirring with a magnetic stirrer, add 30 mL of 0.05 M edetate disodium VS from a 50-mL buret, followed by 15 mL of 1 N sodium hydroxide and 300 mg of hydroxy naphthol blue, and continue the titration to the blue endpoint. Each mL of 0.05 M edetate disodium is equivalent to 13.51 mg of C10H14CaO6.
Auxiliary Information—
Staff Liaison :
Lawrence Evans, III, Ph.D., Scientist
Expert Committee : (DSN05) Dietary Supplements - Non-Botanicals
USP29–NF24 Page 358
Pharmacopeial Forum : Volume No. 27(6) Page 3260
Phone Number : 1-301-816-8389
;)
